Reproduction Abstracts

Although mammalian embryo culture is important for research in early development as well as reproduction engineering, rat embryo culture is still infant other than that of mice. The aim of the present study was to improve rat embryo culture in vitro, we compared modified rat-one cell embryo cuture medium (R1ECM) with potassium simplex optimized medium with amino acids (KSOMaa) and that effect of addition of EDTA (0.013 mM) and glutamine (1 mM) to mR1ECM on develop to ...

In recent years, advances in molecular biology and reproductive engineering make generation of knockout (KO) animals possible. KO animals are useful and powerful tools for agriculture as well as biomedical research. Zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN) genome editing technology enables site directed engineering of the genome. The aim of the present study was to ZFN and TALEN system could work on editing genome of cultured porcin...

The aim of the present study was to evaluate fertility of vitrified rat matured-oocytes with surrounding cumulus cells after IVF with cryopreserved sperm. Oocytes with surrounding cumulus cells and cumulus cells denuded oocytes were vitrified with 30% (v/v) ethylene glycol+0.5 M sucrose+20% (v/v) FCS in PBS (PB1) by using Cryotop. After warming, oocytes were co-cultured with fresh or cryopreserved epididymal sperm for 10 h. Although the fertilization (two pronuclei formation: ...

Although GH receptor (GHR) gene deletion causes a hereditary dwarfism and also shows a longer lifespan, the fact was shown only in mice. Because it was difficult to generate knockout (KO) animals other than mice. Recently, the generation of KO animals became possible without difficulty due to advances in genome editing technologies such as transcription activator-like effector nuclease (TALEN). In the present study, we examined the effect of cRNA concentration microinjected cy...